human genome u133 plus 2.0 microarray platform  (Thermo Fisher)

Journal: Journal of Oncology

Article Title: Identifying Stage II Colorectal Cancer Recurrence Associated Genes by Microarray Meta-Analysis and Building Predictive Models with Machine Learning Algorithms

doi: 10.1155/2021/6657397

Figure Lengend Snippet: Characteristics of the included datasets.

Article Snippet: The majority of the datasets adopted the GPL570 microarray platform (Affymetrix Human Genome U133 Plus 2.0 Array), and 1 dataset adopted the GPL5175 microarray platform (Affymetrix Human Exon 1.0 ST Array), as shown in .

Techniques: Microarray

Journal: Pediatric Rheumatology Online Journal

Article Title: Mitochondrial and oxidative stress genes are differentially expressed in neutrophils of sJIA patients treated with tocilizumab: a pilot microarray study

doi: 10.1186/s12969-016-0067-7

Figure Lengend Snippet: Genes associated with oxidative phosphorylation (mitochondria function). a Enrichment plot of KEGG_Oxidative phosphorylation gene set identified by GSEA. Middle section (black bars) illustrate the position of the genes belonging to the gene set in the context of all the genes on the Affymetrix U133 plus 2.0 array. The enrichment score (ES) plotted as a function of the position within the ranked list of array genes is shown as a green line. The ranked list metric shown in gray illustrates the correlation between the signal to noise values of all individually ranked genes according to the neutrophil samples of the ‘before’ and ‘after’ tocilizumab treatment (experimental conditions). b on the left is GSEA-derived heat map of the 110 leading edge genes that correlates with response to tocilizumab and contributing to the enrichment score; on the right is the top 20 genes that includes some of the genes mapped by IPA to mitochondria dysfunction pathway (in red asterisks). Signal intensities are illustrated by varying shades of red (increased) and blue (decreased). c Quantitative real-time polymerase chain reaction (qRT-PCR) validation of differentially expressed genes ( NDUFB2 , COX6C , LAT ) observed in this microarray experiment. The relative fold change of both microarray (solid bar) and qRT-PCR (open bars) are shown. The data for qRT-PCR are the average of 3 independent experiments done on the same sample

Article Snippet: RNA was extracted and gene expression profiling was performed using Affymetrix GeneChip Human Genome U133 Plus 2.0 microarray platform.

Techniques: Derivative Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Microarray